- Importance Of Serial Dilution And Antibody Titration In Serology
- Importance Of Serial Dilution In Serology
- Serial Dilution
A serial dilution is the stepwise of a in. Usually the at each step is constant, resulting in a of the in a fashion.
A ten-fold serial dilution could be 1, 0.1 M, 0.01 M, 0.001 M. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for resulting in with a. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10 0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10 0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution.
Serial dilutions are widely used in experimental sciences, including, and. Contents.
In biology and medicine In and, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. As, for instance, the number and size of that grow on an plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results. Serial dilution is also a cheaper and simpler method for preparing than and. In homeopathy. Experiments in Microbiology, Plant Pathology and Biotechnology.
New Age Publishers, 2005, p. Booth, C.; et al. Methods in microbiology 35.
Importance Of Serial Dilution And Antibody Titration In Serology
Academic Press. Weissmann, Gerald (2006). The FASEB Journal. 20 (11): 1755–1758.
Retrieved 2008-02-01. Ernst, Edzard (November 2005). 'Is homeopathy a clinically valuable approach?' Trends in Pharmacological Sciences. 26 (11): 547–548. Bishop, Edward P. Fody, Larry E.
Clinical Chemistry: Principles, Procedures, Correlations. Lippincott Williams & Wilkins, 2004, p. 24. External links., Bates College.
Importance Of Serial Dilution In Serology
This article does not any. Unsourced material may be challenged and. (January 2009) An antibody is a measurement of how much an organism has produced that recognizes a particular, expressed as the inverse of the greatest dilution (in a ) that still gives a positive result. Is a common means of determining antibody titers.
Serial Dilution
For example, the detects the presence of anti-Rh antibodies in a pregnant woman's. A patient might be reported to have an 'indirect Coombs titer' of 16. This means that the patient's serum gives a positive indirect Coombs test at any dilution down to 1/16 (1 part serum to 15 parts diluent). At greater dilutions the indirect Coombs test is negative. If a few weeks later the same patient had an indirect Coombs titer of 32 (1/32 dilution which is 1 part serum to 31 parts diluent), this would mean that she was making more anti-Rh antibody, since it took a greater dilution to abolish the positive test. Many traditional tests such as or employ this principle. Such tests can typically be read visually, which makes them fast and cost-effective in a 'low-tech' environment.
The interpretation of serological titers is guided by that are specific to the or in question; a titer of 1:32 may be below the cut-off for one test but above for another.